Syllabus

B.Tech Biotechnology Syllabus 2025 – Latest Semester-Wise Course Layout & PDF

Get the complete B.Tech Biotechnology syllabus 2025 with semester-wise subject details, core topics, lab work, elective options, marks distribution, and project components. Download the official syllabus PDF and check recommended books, specializations, and preparation tips.

B.Tech in Biotechnology is an interdisciplinary engineering program that combines biological sciences with engineering principles to develop technologies in healthcare, agriculture, environment, and industry. This 4-year undergraduate degree is ideal for students interested in genetic research, pharmaceuticals, bioinformatics, and industrial processes involving living organisms.

The B.Tech Biotechnology syllabus is structured over eight semesters, integrating core biotechnology topics with hands-on laboratory practices, electives, industry internships, and project work. This comprehensive guide covers the semester-wise syllabus, topic breakdowns, marks allocation, recommended books, preparation strategies, specializations, and more.

B.Tech Biotechnology Course Overview

PARTICULARS DETAILS
Course Name Bachelor of Technology in Biotechnology
Duration 4 Years (8 Semesters)
Eligibility 10+2 with Physics, Chemistry, and Biology/Mathematics
Admission Process JEE Main / State Entrance Exams / University-based Entrance
Academic Structure Theory + Lab Practicals + Projects + Internship
Major Areas Biochemistry, Molecular Biology, Genetic Engineering, Bioprocess Engineering, Immunology

Semester-wise B.Tech Biotechnology Syllabus with Marks

Semester 1

SUBJECT TOPICS COVERED MARKS
Engineering Mathematics I Differentiation, Integration, Linear Algebra, Differential Equations 100
Physics Mechanics, Oscillations, Thermodynamics, Optics 100
Chemistry Atomic Structure, Bonding, Organic Chemistry Basics 100
Introduction to Biotechnology Definition, History, Applications in Industry and Medicine 100
Communication Skills Verbal & Written Skills, Report Writing, Presentation 50
Lab Practicals Physics Lab, Chemistry Lab, Biotechnology Intro Lab 100

Semester 2

SUBJECT TOPICS COVERED MARKS
Engineering Mathematics II Matrices, Vector Calculus, Complex Numbers, Laplace Transforms 100
Cell Biology Cell Theory, Membrane Structure, Cell Cycle, Mitosis & Meiosis 100
Environmental Science Ecology, Sustainable Development, Environmental Pollution 100
Programming Fundamentals C/C++, Data Structures, Algorithms for Bioinformatics 100
Engineering Mechanics Statics, Dynamics, Equilibrium, Forces 100
Lab Practicals Cell Biology Lab, Programming Lab 100

Semester 3

SUBJECT TOPICS COVERED MARKS
Molecular Biology DNA/RNA Structure, Central Dogma, Replication, Transcription, Translation 100
Biochemistry Proteins, Enzymes, Lipids, Nucleotides, Metabolism 100
Microbiology Microbial Types, Growth, Sterilization, Antibiotics 100
Genetics Mendelian Genetics, Genetic Mapping, Chromosomal Disorders 100
Biotech Instrumentation UV-Vis Spectroscopy, Centrifugation, Microscopy 100
Lab Practicals Microbiology and Biochemistry Lab 100

Semester 4

SUBJECT TOPICS COVERED MARKS
Immunology Innate & Adaptive Immunity, Antigens, Antibodies, Vaccines 100
Bioinformatics FASTA, BLAST, Sequence Alignment, Database Tools 100
Enzyme Technology Enzyme Kinetics, Immobilization, Industrial Applications 100
Structural Biology X-ray Crystallography, Protein/Nucleic Acid Structure 100
Biostatistics Descriptive Stats, Probability, Hypothesis Testing 100
Lab Practicals Immuno & Bioinformatics Lab 100

Semester 5

SUBJECT TOPICS COVERED MARKS
Genetic Engineering rDNA, PCR, Cloning, Vectors 100
Bioprocess Engineering Fermentation, Bioreactors, Sterilization 100
Plant Biotechnology Tissue Culture, Transgenic Plants, Gene Transfer 100
Animal Biotechnology Stem Cells, IVF, Hybridoma Technology 100
Elective I Nanobiotechnology / Systems Biology / Neuroscience 100
Lab Practicals Genetic Engineering Lab, Bioprocess Lab 100

Semester 6

SUBJECT TOPICS COVERED MARKS
Industrial Biotechnology Biofertilizers, Biopolymers, Biofuels 100
Medical Biotechnology Diagnostics, Gene Therapy, Monoclonal Antibodies 100
Protein Engineering Protein Modeling, Drug Design, Protein Folding 100
Bioprocess Control Automation, Control Loops, Sensors in Biotech 100
Elective II Stem Cell Technology / IPR / Genomics 100
Lab Practicals Medical Biotech & Protein Engineering Lab 100

Semester 7

SUBJECT TOPICS COVERED MARKS
Bioreactor Design Types of Bioreactors, Scale-up, Reactor Modeling, Kinetics 100
Bioethics & Biosafety GMO Guidelines, Biosafety Levels, Ethical Research Practices 100
Elective III Environmental Biotechnology / Metabolomics / Synthetic Biology 100
Industrial Training / Seminar Training Report or Seminar on Industrial Visit or Biotech Trend 100
Project Phase I Literature Review, Objective Setting, Research Proposal 100

Semester 8

SUBJECT TOPICS COVERED MARKS
Project Phase II Experimental Work, Data Analysis, Thesis Report, Viva 200
Internship / Dissertation 6–8 weeks of Research or Industry-based Internship 100
Comprehensive Viva Oral Examination covering entire B.Tech curriculum 100

B.Tech Biotechnology Syllabus PDF

Download the official B.Tech Biotechnology Syllabus PDF here: [Download PDF]

Recommended Books for B.Tech Biotechnology

SUBJECT BOOK AUTHOR
Molecular Biology Molecular Biology of the Cell Bruce Alberts
Biochemistry Lehninger Principles of Biochemistry Nelson & Cox
Genetics Genetics: A Conceptual Approach Benjamin A. Pierce
Microbiology Prescott’s Microbiology Willey et al.
Bioprocess Engineering Bioprocess Engineering Principles Pauline M. Doran
Bioinformatics Bioinformatics: Sequence Analysis David Mount
Immunology Kuby Immunology Kindt & Goldsby

Preparation Tips

  • Start with basic biology and chemistry concepts in the first year.
  • Create mind maps and flowcharts for complex pathways and processes.
  • Practice lab work thoroughly — this is a practical-heavy course.
  • Use NPTEL video lectures for topics like Bioprocess or Immunology.
  • Stay updated with recent biotech news via journals like Nature Biotechnology.
  • Participate in biotech internships and seminars for exposure.

Specializations After B.Tech Biotechnology

SPECIALIZATION DESCRIPTION
Genetic Engineering Focus on DNA modification, cloning, gene editing
Bioinformatics Computational analysis of biological data
Medical Biotechnology Healthcare applications like diagnostics and therapeutics
Industrial Biotechnology Large-scale production of bio-products
Stem Cell Technology Research on regenerative medicine and organ development
Environmental Biotechnology Biotech solutions for waste treatment and pollution control

Conclusion

The B.Tech Biotechnology syllabus is designed to empower students with a strong foundation in biology, engineering, and research methodology. It opens doors to careers in healthcare, pharma, research labs, and global biotech firms. With proper understanding, practical exposure, and academic discipline, students can succeed both in academics and in advanced research domains.

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